利用死后人体突触体进行代谢及递质氨基酸释放的研究。

Use of post-mortem human synaptosomes for studies of metabolism and transmitter amino acid release.

作者信息

Hardy J A, Dodd P R, Oakley A E, Kidd A M, Perry R H, Edwardson J A

出版信息

Neurosci Lett. 1982 Dec 13;33(3):317-22. doi: 10.1016/0304-3940(82)90392-5.

Abstract

Synaptosomes have been prepared from human brain obtained at autopsies carried out up to 24 h postmortem (p.m.). They showed generally good retention of morphology, as well as accumulation of tissue potassium and linear rates of oxygen uptake. In response to veratrine depolarization they showed increased respiration rate, decreased tissue potassium content and the specific release of transmitter amino acids. Regression analysis indicated that metabolically and functionally active preparations may be obtained up to ca. 25 h p.m. Preparations obtained from patients dying with brain injury were inactive.

摘要

突触体是从尸检时获取的人脑中制备的，尸检时间为死后（p.m.）24小时以内。它们通常显示出良好的形态保留，以及组织钾的积累和线性耗氧率。对藜芦碱去极化的反应中，它们显示出呼吸速率增加、组织钾含量降低以及递质氨基酸的特定释放。回归分析表明，在大约死后25小时内可以获得代谢和功能活跃的制剂。从死于脑损伤的患者获得的制剂没有活性。