Korenaga S, Kou K, Ito Y
Proc R Soc Lond B Biol Sci. 1982 Dec 22;217(1206):89-99. doi: 10.1098/rspb.1982.0096.
The effects of denervation on the electrical membrane properties of fish red muscle were investigated. Forty to fifty hours after denervation, miniature endplate potentials disappeared abruptly and field stimulation of the nerve within the muscle failed to evoke endplate potentials, indicating that transmission failure occurred at this time. The membrane resistance of the red muscle fibre increased after denervation. Normally innervated fish red muscles do not generate action potentials in response to either nerve or direct muscle stimulation. However, approximately 3 weeks after nerve sectioning, action potentials could be induced in the muscles. The action potential was sodium-dependent, and was sensitive to tetrodotoxin. Actinomycin D injected in the early phase after operation suppressed the induction of the action potential. These results indicate that RNA synthesis is preliminary to the induction of the action potential mechanism, and that this mechanism is under neural control.
研究了去神经支配对鱼类红色肌肉电膜特性的影响。去神经支配40至50小时后，微小终板电位突然消失，对肌肉内神经的场刺激未能诱发终板电位，表明此时发生了传递失败。去神经支配后红色肌纤维的膜电阻增加。正常支配的鱼类红色肌肉对神经或直接肌肉刺激均无动作电位产生。然而，在神经切断后约3周，肌肉中可诱发出动作电位。该动作电位依赖于钠，对河豚毒素敏感。术后早期注射放线菌素D可抑制动作电位的诱导。这些结果表明，RNA合成是动作电位机制诱导的前提，且该机制受神经控制。
