Borgatti A R, Trigari G, Pagliarani A, Ventrella V
Boll Soc Ital Biol Sper. 1982 Dec 15;58(23):1516-22.
Arrhenius plot of glutamate, succinate and ascorbate+TMPD oxidation in bass liver mitochondria show a break at different temperatures. Above the break activation energies (Ea) of the three enzymes. Above the break activation energies (Ea) of the three enzymes examined are similar and comparable with literature data in poikilotermic and homeothermic animals. Below the break the Ea are again comparable with poikiloterm and homeotherm ones except for succinate-oxidase whose Ea is surprisingly higher. The data are suggested to be due to the features of the enzymes or to the microenvironmental physical state.
鲈鱼肝脏线粒体中谷氨酸、琥珀酸以及抗坏血酸+四甲基对苯二胺氧化反应的阿累尼乌斯曲线在不同温度下出现转折。转折温度以上,所检测的三种酶的活化能(Ea)相似,且与变温动物和恒温动物的文献数据相当。转折温度以下,除琥珀酸氧化酶的活化能出奇地高之外,其余酶的活化能再次与变温动物和恒温动物的相当。这些数据表明是由于酶的特性或微环境物理状态所致。