Klug E, Deegen E, Lazarz B, Rojem I, Merkt M
J Reprod Fertil Suppl. 1982;32:31-4.
Successful empirical treatment of 17 out of 24 stallions, which had failed to ejaculate after normal penile erection, intromission and friction, by chemical blockage of beta-receptors and additional stimulation of alpha-receptors led us to investigate stallions with normal ejaculatory patterns. In an initial experiment one adult half-bred stallion was injected with 4.88 mg noradrenaline hydrochloride (treatment A), 10 mg bunitrolol (treatment B = beta-receptor blockage) and a combination of treatment A and B. Investigations of the same stallion on dated occasions without treatment served as controls. The test criteria were heart rate during copulation by telemetric measurement, volume of ejaculates and sperm concentration per ml and per ejaculate. In a second experiment, 2 stallions were treated after a modified test schedule. In addition to the criteria used in the first experiment seminal citric acid, ergothioneine, and glycerophosphocholine were measured. Administration of the beta-blocker alone or in combination with alpha-receptor stimulation resulted in (a) significant lowering of the maximal heart rate, (b) significant increase of sperm count per ejaculate and (c) significant rise of glycerophosphocholine.
24匹种马在阴茎正常勃起、插入和摩擦后未能射精，其中17匹通过β受体化学阻断和α受体额外刺激成功进行了经验性治疗，这促使我们对射精模式正常的种马展开研究。在首个实验中，给一匹成年混血种马注射了4.88毫克盐酸去甲肾上腺素（治疗A）、10毫克布尼洛尔（治疗B = β受体阻断）以及治疗A和B的组合。在未进行治疗的不同日期对同一匹种马进行调查作为对照。测试标准包括通过遥测测量交配期间的心率、射精量以及每毫升和每次射精的精子浓度。在第二个实验中，按照修改后的测试方案对2匹种马进行了治疗。除了首个实验中使用的标准外，还测量了精液中的柠檬酸、麦角硫因和甘油磷酸胆碱。单独给予β受体阻滞剂或与α受体刺激联合使用导致：（a）最大心率显著降低；（b）每次射精的精子数量显著增加；（c）甘油磷酸胆碱显著升高。
