Astrup E G, Iversen O H
Virchows Arch B Cell Pathol Incl Mol Pathol. 1983;42(1):1-18. doi: 10.1007/BF02890365.
It is known that a single application of 17 nmol 12-O-tetradecanoylphorbol-13-acetate (TPA) to hairless mouse skin first induces (from 0-12 h) a short block in mitotic activity followed by a transient stimulation of proliferation (from 12-96 h) characterized by multiple waves of rapid proliferation in partly synchronized basal cells, leading to hyperplasia. The replication rate of basal cells, the number of basal and suprabasal (maturing) cells per unit of interfollicular epidermis and the number of squamous layers in the stratum corneum were recorded. Changes in the nuclear area of living cells were monitored by morphometry. The changes in the rate of basal cell proliferation are accompanied by concomitant waves of increased rates of cell maturation and cell loss, with a considerable reduction in epidermal cell transit time. When 14-15 squamous layers were observed, this resulted in an increased cell loss, which was visible as scaling from 24 h onwards. The total cell mass lost from 16 to 72 h after TPA application amounted to about 67% of the newborn cell mass. Thus the hyperplasia occurring after a single TPA treatment results from a considerably enhanced cell proliferation that exceeds concomitant increases in the rates of cell maturation and loss. There is no delayed maturation. The results are also consistent with the chalone theory of epidermal growth regulation, assuming that the G1 chalone is produced during cell maturation.
已知对无毛小鼠皮肤单次施用17 nmol 12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA），首先会在有丝分裂活性方面诱导一个短暂阻滞（0 - 12小时），随后是增殖的短暂刺激（12 - 96小时），其特征是部分同步化的基底细胞出现多波快速增殖，导致增生。记录了基底细胞的复制速率、单位毛囊间表皮中基底细胞和基底上层（正在成熟的）细胞的数量以及角质层中的鳞状层数。通过形态测量法监测活细胞的核面积变化。基底细胞增殖速率的变化伴随着细胞成熟速率和细胞丢失速率的相应增加，表皮细胞转运时间显著缩短。当观察到14 - 15层鳞状层时，这导致细胞丢失增加，从24小时起可见脱屑现象。TPA施用后16至72小时内丢失的总细胞量约占新生细胞量的67%。因此，单次TPA处理后出现的增生是由于细胞增殖显著增强，超过了细胞成熟速率和丢失速率的相应增加。不存在成熟延迟现象。这些结果也与表皮生长调节的抑素理论一致，假定G1抑素是在细胞成熟过程中产生的。
