α-和β-肾上腺素能受体激动剂以及磷酸二酯酶抑制剂（ICI 63 197）对牛脾神经中环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）水平的影响。

Effects of alpha- and beta-adrenoceptor agonists and a phosphodiesterase inhibitor (ICI 63 197) on cyclic AMP and GMP levels in bovine splenic nerve.

Merican Z, Nott M W

Clin Exp Pharmacol Physiol. 1983 Jan-Feb;10(1):35-44. doi: 10.1111/j.1440-1681.1983.tb00169.x.

Abstract

Cyclic AMP and cyclic GMP levels were determined in bovine splenic nerve segments in the absence and presence of (+/-)-isoprenaline, (-)-phenylephrine, clonidine and ICI 63 197 (a phosphodiesterase inhibitor). The chosen concentrations of adrenoceptor agonists were those which are known to affect stimulation-induced overflow of noradrenaline from nerve terminals. 2. The mean levels of cyclic AMP ranged from 229 to 555 pmol/g of microwave irradiated tissue. Mean cyclic GMP levels ranged from 27.9 to 42.2 pmol/g. 3. Isoprenaline enhanced cyclic AMP levels but did not affect cyclic GMP levels. The effect was blocked with (+/-)-propranolol. ICI 63 197 increased cyclic AMP levels but did not change cyclic GMP. Phenylephrine and clonidine caused no consistent changes in cyclic AMP or cyclic GMP levels or in the concentration ratio between these two nucleotides. 4. The results support the involvement of cyclic AMP in the enhancing effect of beta-adrenoceptor agonists and phosphodiesterase inhibitors on stimulation-induced release of noradrenaline from sympathetic nerves.

摘要

在不存在和存在（±）-异丙肾上腺素、（-）-去氧肾上腺素、可乐定和ICI 63197（一种磷酸二酯酶抑制剂）的情况下，测定了牛脾神经节段中的环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）水平。所选用的肾上腺素能受体激动剂浓度是已知会影响刺激诱导的去甲肾上腺素从神经末梢溢出的浓度。2. 微波照射组织中cAMP的平均水平在229至555 pmol/g之间。cGMP的平均水平在27.9至42.2 pmol/g之间。3. 异丙肾上腺素提高了cAMP水平，但不影响cGMP水平。该作用被（±）-普萘洛尔阻断。ICI 63197提高了cAMP水平，但未改变cGMP。去氧肾上腺素和可乐定对cAMP或cGMP水平或这两种核苷酸之间的浓度比没有一致的影响。4. 这些结果支持cAMP参与β-肾上腺素能受体激动剂和磷酸二酯酶抑制剂对刺激诱导的交感神经去甲肾上腺素释放的增强作用。