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Criteria for the standardization of Salmonella mutagenicity tests: results of a collaborative study. III. The influence of the composition and preparation of the minimal medium in the Salmonella mutagenicity test.

作者信息

Aeschbacher H U, Friederich U, Seiler J P

出版信息

Teratog Carcinog Mutagen. 1983;3(2):195-203. doi: 10.1002/1520-6866(1990)3:2<195::aid-tcm1770030212>3.0.co;2-q.

DOI:10.1002/1520-6866(1990)3:2<195::aid-tcm1770030212>3.0.co;2-q
PMID:6133374
Abstract

The influence of factors connected with the preparation of the minimal medium for the Ames test has been investigated. Faulty sterilization procedures can lead to the generation of toxic and/or mutagenic by-products in the minimal medium. Changes in histidine concentration affect not only the number of spontaneously arising colonies on the plate, but also the number of induced mutants. Although the number of spontaneous mutants increases slightly with increasing histidine concentration, the influence on the number of induced mutants depends on the nature of the mutagen tested.

摘要

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