Cille Y, Deviller P, Betuel H
Enzyme. 1983;29(2):86-92. doi: 10.1159/000469612.
The subcellular repartition and the distinctive properties of guanylate cyclase (EC 4.6.1.2) vary according to the lymphocyte population studied and according to the presence of detergent. Guanylate cyclase of non-adherent peripheral lymphocytes and of thymus lymphocytes is recovered by more than 90% in the soluble fraction of the homogenate. Kinetics according to the substrate (5'-GTP-Mn2+) is Michaelian, the Ca2+ ion acts as an activator, especially in the case of blood lymphocytes, and the detergent has no effect on the enzyme activity. On the other hand, the guanylate cyclase of tonsil lymphocytes reside in the particulate fraction. It has non-Michaelian kinetics for the substrate, a strong stimulating effect of detergent, and an inhibitory effect of Ca2+. A comparison of the enzymatic activities of unseparated and of non-adherent tonsil lymphocytes obtained from the same donor points to a correlation between their T and B properties: predominant soluble activity in the T population and particulate guanyl cyclase activity in the B subset.
鸟苷酸环化酶（EC 4.6.1.2）的亚细胞分布及其独特性质会因所研究的淋巴细胞群体以及去污剂的存在与否而有所不同。非黏附性外周淋巴细胞和胸腺淋巴细胞中的鸟苷酸环化酶，在匀浆的可溶部分中回收率超过90%。以底物（5'-GTP-Mn2+）为依据的动力学呈米氏动力学，Ca2+离子起激活剂作用，尤其是在血液淋巴细胞的情况下，去污剂对酶活性无影响。另一方面，扁桃体淋巴细胞中的鸟苷酸环化酶存在于颗粒部分。它对底物具有非米氏动力学，去污剂有强烈刺激作用，Ca2+有抑制作用。对来自同一供体的未分离扁桃体淋巴细胞和非黏附性扁桃体淋巴细胞的酶活性进行比较，结果表明它们的T和B特性之间存在相关性：T群体中主要是可溶活性，B亚群中是颗粒性鸟苷酸环化酶活性。
