Smith D D, Vorhaben J E, Campbell J W
J Exp Zool. 1983 Apr;226(1):29-35. doi: 10.1002/jez.1402260105.
Rabbit antibody to chicken liver mitochondrial glutamine synthetase was purified by immunoaffinity chromatography for analysis of the immunological relatedness of vertebrate glutamine synthetases. The antibody cross-reacted with enzymes from representatives of all five vertebrate classes, indicating a high degree of evolutionary conservatism in the structure of the enzymes. A unique aspect of the immunological similarity of these enzymes is that it exists between cytosolic and mitochondrial enzymes which are, in general, immunologically distinct. The antibody did not cross-react with two insect glutamine synthetases. Compositional difference indices, calculated from the amino acid compositions of glutamine synthetases from several species, gave a mean estimate of over 80% sequence homology for the vertebrate enzymes. The avian mitochondrial enzyme gave a mean 78% homology with the mammalian cytosolic enzyme.
通过免疫亲和层析纯化了兔抗鸡肝线粒体谷氨酰胺合成酶抗体，用于分析脊椎动物谷氨酰胺合成酶的免疫相关性。该抗体与所有五类脊椎动物代表的酶发生交叉反应，表明这些酶的结构具有高度的进化保守性。这些酶免疫相似性的一个独特之处在于，它存在于通常在免疫学上不同的胞质和线粒体酶之间。该抗体与两种昆虫谷氨酰胺合成酶不发生交叉反应。根据几种物种谷氨酰胺合成酶的氨基酸组成计算的组成差异指数表明，脊椎动物酶的平均序列同源性估计超过80%。禽类线粒体酶与哺乳动物胞质酶的平均同源性为78%。
