Morel A, Nicolas P, Cohen P
J Biol Chem. 1983 Jul 10;258(13):8273-6.
This paper demonstrates the presence of a single predominant form of high molecular weight somatostatin (Mr congruent to 15,000) in mouse hypothalamus as judged by a combination of purification procedures including molecular sieve filtration, reverse phase high pressure liquid chromatography, isoelectric focusing, and gel electrophoresis under denaturing conditions. Furthermore, the conversion of the Mr = 15,000 somatostatin species by hypothalamic enzyme activities led to the formation of both somatostatin-14 and -28 which were identified by high pressure liquid chromatography. No evidence was found for the presence of molecular species containing the sequence of the (Tyr-7, Gly-10) derivative of somatostatin. These observations suggest that, in contrast to the situation encountered in the pancreas, a predominant form of prosomatostatin is synthesized in hypothalamic neurones. They indicate that, in the hypothalamus, both the tetradecapeptide and somatostatin-28 derive from a single prohormone.
本文通过包括分子筛过滤、反相高压液相色谱、等电聚焦以及变性条件下的凝胶电泳等一系列纯化程序，证明小鼠下丘脑存在单一主要形式的高分子量生长抑素（分子量约为15,000）。此外，下丘脑酶活性可使分子量为15,000的生长抑素物种转化为生长抑素-14和-28，这两种产物通过高压液相色谱得以鉴定。未发现存在含有生长抑素（Tyr-7，Gly-10）衍生物序列的分子物种的证据。这些观察结果表明，与胰腺中遇到的情况相反，下丘脑神经元合成了一种主要形式的前生长抑素。它们表明，在下丘脑中，十四肽和生长抑素-28均源自单一前体激素。
