Daldrup T
Z Rechtsmed. 1983;90(1):19-25. doi: 10.1007/BF01886063.
In an experiment a corpse had been kept at room temperature (16 degrees-23 degrees C) for 1163 h. At regular intervals brain samples were taken and the content of free amino acids and related compounds was determined by column chromatography. It could be demonstrated that in a period of 4-20 days postmortem the age of the corpse could be calculated from the concentrations of alpha-aminobutyrate (ABU), gamma-aminobutyrate (GABA), and glutamic acid (GLU) in brain employing the previously [5] presented formula: (formula; see text) T is the postmortem time lapse (days). ABU, GABA, and GLU are the concentrations (mumol/g) wet tissue of the corresponding amino acids. During this postmortem interval there is nearly a linear correlation; from this interval a correct assignment between concentrations and time cannot be given. The determination method is in the range of about 15 degrees-25 degrees C (guarantees bacterial growth and metabolism) independent of ambient temperature. The brain samples (cortex or parts of the putrified brain mush) can be taken without any special precaution during normal autopsy. The results of the experiment (in Fig. 1) were supported by some practical cases where the time lapse since death was well (in Fig. 1) or reasonably (in Fig. 1) known. These results suggest that this method allows in many cases the determination of the age of a corpse found in a warm environment (approximately 15 degrees-25 degrees C) approximately 4-20 days after death.
在一项实验中，一具尸体在室温（16摄氏度至23摄氏度）下保存了1163小时。每隔一段时间采集脑样本，并通过柱色谱法测定游离氨基酸及相关化合物的含量。结果表明，在死后4至20天内，利用先前[5]给出的公式，可根据脑中α-氨基丁酸（ABU）、γ-氨基丁酸（GABA）和谷氨酸（GLU）的浓度计算尸体的死亡时间：（公式；见原文）T为死后时间间隔（天）。ABU、GABA和GLU为相应氨基酸在湿组织中的浓度（微摩尔/克）。在此死后时间间隔内，几乎呈线性相关；但从此时间间隔无法给出浓度与时间之间的准确对应关系。该测定方法在约15摄氏度至25摄氏度范围内（保证细菌生长和代谢）与环境温度无关。在正常尸检过程中，无需采取任何特殊预防措施即可采集脑样本（大脑皮层或部分腐败的脑糊）。实验结果（图1）得到了一些实际案例的支持，在这些案例中，死亡后的时间间隔已知（图1中为确切已知，图1中为大致已知）。这些结果表明，该方法在许多情况下能够确定在温暖环境（约15摄氏度至25摄氏度）中死后约4至20天发现的尸体的死亡时间。
