大西洋鳕鱼（Gadus morhua）嗜铬组织中儿茶酚胺的体外和体内生物合成。

Catecholamine biosynthesis in vitro and in vivo in the chromaffin tissue of the Atlantic cod, Gadus morhua.

Jönsson A C, Nilsson S

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1983;75(1):43-6. doi: 10.1016/0742-8413(83)90009-9.

Abstract

The biosynthesis of [3H]catecholamines from [3H]L-tyrosine in the intact chromaffin tissue of cod posterior cardinal veins was studied in vitro and in vivo at 10 degrees C. 2. The tritiated products dopamine, noradrenaline and adrenaline were separated from the [3H]tyrosine by paper chromatography of tissue extracts and the radioactivity of 1 cm strips of the chromatogram was determined by liquid scintillation spectrophotometry. DOPA could never be demonstrated in the tissue extracts from any of the experiments performed. 3. The content of [3H]noradrenaline in pieces of the cardinal veins incubated in vitro was found to increase rapidly. The tissue content of dopamine and adrenaline remained at lower levels which were reached during the first few hours of the incubation. A similar pattern could be demonstrated in the chromaffin tissue in vivo after infusion of [3H]tyrosine, but the total content of the [3H]catecholamines was lower than in the in vitro experiments. 4. The results are consistent with the view that the methylation of noradrenaline is the rate-limiting step in the biosynthesis of adrenaline in cod chromaffin tissue.

摘要

在10摄氏度下，对鳕鱼后主静脉完整嗜铬组织中由[3H]L-酪氨酸生物合成[3H]儿茶酚胺进行了体外和体内研究。2. 通过组织提取物的纸层析将氚化产物多巴胺、去甲肾上腺素和肾上腺素与[3H]酪氨酸分离，并用液体闪烁分光光度法测定层析图谱1厘米条带的放射性。在进行的任何实验的组织提取物中都从未检测到多巴。3. 发现在体外孵育的主静脉片段中[3H]去甲肾上腺素的含量迅速增加。多巴胺和肾上腺素的组织含量保持在较低水平，这是在孵育的最初几个小时内达到的。在注入[3H]酪氨酸后，体内嗜铬组织也可显示出类似模式，但[3H]儿茶酚胺的总含量低于体外实验。4. 这些结果与以下观点一致，即去甲肾上腺素的甲基化是鳕鱼嗜铬组织中肾上腺素生物合成的限速步骤。