Dyviniak S D, Dunham V L
Biochem Biophys Res Commun. 1983 Jul 29;114(2):604-11. doi: 10.1016/0006-291x(83)90823-9.
Glutamine synthetase (GS) activity recovered from linear sucrose gradients was associated with the cytosol of cells isolated from etiolated soybean hypocotyls whereas light-grown tissue contained increased GS activity localized in both the cytosol and chloroplasts. DEAE-cellulose chromatography indicated two GS isoforms in etiolated hypocotyls whereas light-grown hypocotyls and primary leaves contained four isoforms. Only one GS isoform was recovered from both etiolated and light-grown cotyledons.
从线性蔗糖梯度中回收的谷氨酰胺合成酶（GS）活性与从黄化大豆下胚轴分离的细胞的胞质溶胶相关，而光生长组织中，胞质溶胶和叶绿体中的GS活性均增加。二乙氨基乙基纤维素色谱法表明，黄化下胚轴中有两种GS同工型，而光生长下胚轴和初生叶中有四种同工型。从黄化和光生长的子叶中均只回收了一种GS同工型。
