Kuno T, Matsuda Y, Katunuma N
Biochem Biophys Res Commun. 1983 Jul 29;114(2):889-95. doi: 10.1016/0006-291x(83)90864-1.
The subcellular distributions of the precursor form and mature form of gamma-glutamyltranspeptidase of rat kidney were studied by labeling the enzyme with [3H]fucose in vivo. In trans Golgi elements and basolateral membranes, gamma-glutamyltranspeptidase was present as a precursor form with a single polypeptide chain. However, the brush border membranes contained the heavy and light subunits as well as precursor. These results suggest that the precursor is converted to the mature form after its transport to the brush border membranes.
通过在体内用[3H]岩藻糖标记大鼠肾脏γ-谷氨酰转肽酶，研究了该酶前体形式和成熟形式的亚细胞分布。在反式高尔基体元件和基底外侧膜中，γ-谷氨酰转肽酶以单条多肽链的前体形式存在。然而，刷状缘膜含有重链和轻链亚基以及前体。这些结果表明，前体在转运至刷状缘膜后转化为成熟形式。
