Meyne J, Legator M S
Teratog Carcinog Mutagen. 1983;3(3):281-7. doi: 10.1002/1520-6866(1990)3:3<281::aid-tcm1770030307>3.0.co;2-e.
Embryos from day 12 pregnant Swiss mice given intraperitoneal injections of nitrogen mustard (HN2) or cyclophosphamide (CP) were evaluated for chromosomal aberrations. Both agents induced dose-dependent increases in the frequency of cells with aberrations observed in embryos from females treated 6 hr before sacrifice. The highest frequencies of cells with aberrations were observed when females were injected 15 or 18 hr before sacrifice on day 12. A teratogenic dose of HN2 (1.0 mg/kg) induced significantly higher frequencies of damaged cells than a teratogenic dose of CP (20 mg/kg). Cytogenetic analysis of rodent embryos from pregnant females exposed to xenobiotic agents may be an effective screening test for evaluation of genetic effects induced by transplacental exposure.
对怀孕12天的瑞士小鼠胚胎进行腹腔注射氮芥（HN2）或环磷酰胺（CP），然后评估其染色体畸变情况。在处死前6小时接受处理的雌性小鼠胚胎中，两种药物均诱导出现畸变的细胞频率呈剂量依赖性增加。在第12天处死前15或18小时给雌性小鼠注射药物时，观察到出现畸变的细胞频率最高。致畸剂量的HN2（1.0毫克/千克）诱导的受损细胞频率显著高于致畸剂量的CP（20毫克/千克）。对暴露于异生物剂的怀孕雌性啮齿动物胚胎进行细胞遗传学分析，可能是评估经胎盘暴露诱导的遗传效应的一种有效筛选试验。
