兔牙槽骨细胞膜中前列腺素E2受体的一些特性
Some properties of prostaglandin E2 receptors in rabbit alveolar bone cell membranes.
作者信息
Yoshizawa Y, Abiko Y, Takiguchi H
出版信息
Int J Biochem. 1983;15(8):991-5. doi: 10.1016/0020-711x(83)90034-4.
[3H]prostaglandin E2 (PGE2) binding receptors exist in rabbit alveolar bone cell membranes. The presence of high (Kd = 3.9 X 10(-9) M) and low (Kd = 8.8 X 10(-8) M) affinity binding sites of [3H]PGE2 was demonstrated. The saturation values of [3H]PGE2 for high and low affinity binding sites were 0.13 pmol/mg protein and 1.22 pmol/mg protein, respectively. The digestion of the membranes with pronase, phospholipase C, D and neuraminidase led to a decrease of [3H]PGE2 binding but phospholipase A2 did not.
[3H]前列腺素E2（PGE2）结合受体存在于兔牙槽骨细胞膜中。已证实存在[3H]PGE2的高亲和力（Kd = 3.9×10^(-9) M）和低亲和力（Kd = 8.8×10^(-8) M）结合位点。[3H]PGE2在高亲和力和低亲和力结合位点的饱和值分别为0.13 pmol/mg蛋白质和1.22 pmol/mg蛋白质。用链霉蛋白酶、磷脂酶C、D和神经氨酸酶对膜进行消化导致[3H]PGE2结合减少，但磷脂酶A2没有此作用。
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