Burgoyne R D, Geisow M J, Barron J
Proc R Soc Lond B Biol Sci. 1982 Aug 23;216(1202):111-5. doi: 10.1098/rspb.1982.0064.
Stimulation of isolated chromaffin cells with carbamylcholine led to a number of morphological changes, indicative of exocytosis, apparently resulting from translocation of secretory granules to the plasma membrane and their subsequent fusion with the plasma membrane to release their contents. However, stimulation in the presence of trifluoperazine resulted only in the accumulation of secretory granules close to the plasma membrane. Thus exocytosis could be divided into two stages: a trifluoperazine-insensitive stage involving translocation of secretory granules to the plasma membrane and a second trifluoperazine-sensitive stage resulting in granule-plasma membrane fusion.
用氨甲酰胆碱刺激分离的嗜铬细胞会导致许多形态学变化，这表明存在胞吐作用，显然是由于分泌颗粒转移到质膜并随后与质膜融合以释放其内容物所致。然而，在三氟拉嗪存在的情况下进行刺激，只会导致分泌颗粒在质膜附近积累。因此，胞吐作用可分为两个阶段：一个对三氟拉嗪不敏感的阶段，涉及分泌颗粒向质膜的转移；以及第二个对三氟拉嗪敏感的阶段，导致颗粒与质膜融合。
