Glavinović M I, Miledi R, Nakajima Y
Proc R Soc Lond B Biol Sci. 1983 Aug 22;219(1214):91-101. doi: 10.1098/rspb.1983.0061.
Adult frog (Rana temporaria) muscle fibres, denervated in vivo, were dissociated and maintained in culture for several weeks. Light and electron microscopical studies showed that the fibres developed striated muscle sprouts. These sprouts were in cytoplasmic continuity with the parent muscle fibre. To judge by the presence of miniature endplate potentials, embryonic Xenopus laevis neurons were able to form functional neuromuscular junctions, both on the muscle sprouts and on the parent fibre. In addition to the usual depolarizing action potentials, the cultured fibres, with or without sprouts, showed slow hyperpolarizing regenerative responses. These hyperpolarizing action potentials were triggered when the membrane potential reached about -120 mV and their 'peak' amplitude was at about -230 mV. It is concluded that new skeletal muscle fibres can be formed from outgrowths of adult muscle fibres, and that these sprouts can accept motor innervation.
成年青蛙（欧洲林蛙）的肌肉纤维在体内去神经支配后，被分离并在培养中维持数周。光镜和电镜研究表明，这些纤维长出了横纹肌芽。这些芽与母肌纤维的细胞质相连。根据微小终板电位的存在判断，非洲爪蟾胚胎神经元能够在肌芽和母纤维上形成功能性神经肌肉接头。除了常见的去极化动作电位外，有或没有芽的培养纤维都表现出缓慢的超极化再生反应。当膜电位达到约 -120 mV 时，这些超极化动作电位被触发，其“峰值”幅度约为 -230 mV。得出的结论是，新的骨骼肌纤维可以由成年肌纤维的生长形成，并且这些芽可以接受运动神经支配。
