Park C E, Rayman M K, Szabo R, Stankiewicz Z K
Can J Microbiol. 1976 May;22(5):654-7. doi: 10.1139/m76-096.
A procedure involving the use of citrate-buffered lactose broth (pH 6.5) containing an analogue of a beta-galactoside (4-chloro-2-cyclopentylphenyl beta-D-galactopyranoside) has been developed for the enrichment of Shigella in competition with a 100-fold higher population of Escherichia coli. The system makes use of the beta-galactosidase activity of E. coli which hydrolyzes the phenolic derivative of beta-galactoside to galactose and an aglycone moiety (4-chloro-2-cyclopentylphenol) which is toxic to E. coli but is tolerated by Shigella. The procedure is particularly effective in the enrichment of S. sonnei and S. flexneri; S. dynsenteriae and S. boydii are enriched to a lesser extent.
已开发出一种程序，该程序使用含有β-半乳糖苷类似物（4-氯-2-环戊基苯基β-D-吡喃半乳糖苷）的柠檬酸盐缓冲乳糖肉汤（pH 6.5），用于在与数量高100倍的大肠杆菌竞争中富集志贺氏菌。该系统利用大肠杆菌的β-半乳糖苷酶活性，将β-半乳糖苷的酚类衍生物水解为半乳糖和一个糖苷配基部分（4-氯-2-环戊基苯酚），该糖苷配基对大肠杆菌有毒，但志贺氏菌可以耐受。该程序在富集宋内志贺氏菌和福氏志贺氏菌方面特别有效；痢疾志贺氏菌和鲍氏志贺氏菌的富集程度较低。
