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Regulatory mutations in CHO cells induce expression of the mouse embryonic antigen SSEA-1.

作者信息

Campbell C, Stanley P

出版信息

Cell. 1983 Nov;35(1):303-9. doi: 10.1016/0092-8674(83)90233-7.

DOI:10.1016/0092-8674(83)90233-7
PMID:6138159
Abstract

Two rare and dominant mutants of Chinese hamster ovary (CHO) cells, LEC11 and LEC12, express the mouse embryonic antigen SSEA-1. Parental CHO cells and the revertants, LEC11.R9 and LEC12.R10, do not express this antigen as detected by a sensitive radioimmunoassay with a monoclonal antibody to SSEA-1. The presence of the SSEA-1 determinant correlates with the apparent de novo expression of specific N-acetylglucosaminide alpha(1,3)fucosyltransferase activities not detected in parental or revertant cell extracts. Several differences in the enzymes substrate specificities and their products have been identified. The combined data suggest that LEC11 and LEC12 mutants result from regulatory mutations affecting different fucosyltransferase genes.

摘要

相似文献

1
Regulatory mutations in CHO cells induce expression of the mouse embryonic antigen SSEA-1.
Cell. 1983 Nov;35(1):303-9. doi: 10.1016/0092-8674(83)90233-7.
2
The GDP-fucose:N-acetylglucosaminide 3-alpha-L-fucosyltransferases of LEC11 and LEC12 Chinese hamster ovary mutants exhibit novel specificities for glycolipid substrates.LEC11和LEC12中国仓鼠卵巢突变体的GDP-岩藻糖：N-乙酰葡糖胺3-α-L-岩藻糖基转移酶对糖脂底物表现出新颖的特异性。
J Biol Chem. 1987 Dec 15;262(35):16830-7.
3
The Chinese hamster ovary glycosylation mutants LEC11 and LEC12 express two novel GDP-fucose:N-acetylglucosaminide 3-alpha-L-fucosyltransferase enzymes.中国仓鼠卵巢糖基化突变体LEC11和LEC12表达两种新型GDP-岩藻糖：N-乙酰葡糖胺3-α-L-岩藻糖基转移酶。
J Biol Chem. 1984 Sep 25;259(18):11208-14.
4
Activation of two new alpha(1,3)fucosyltransferase activities in Chinese hamster ovary cells by 5-azacytidine.5-氮杂胞苷激活中国仓鼠卵巢细胞中的两种新的α(1,3)岩藻糖基转移酶活性
Cell Regul. 1991 Dec;2(12):989-1000. doi: 10.1091/mbc.2.12.989.
5
alpha(1,3)fucosyltransferases expressed by the gain-of-function Chinese hamster ovary glycosylation mutants LEC12, LEC29, and LEC30.功能获得性中国仓鼠卵巢糖基化突变体LEC12、LEC29和LEC30所表达的α(1,3)岩藻糖基转移酶。
Arch Biochem Biophys. 2000 Mar 15;375(2):322-32. doi: 10.1006/abbi.1999.1693.
6
Transfection of a human alpha-(1,3)fucosyltransferase gene into Chinese hamster ovary cells. Complications arise from activation of endogenous alpha-(1,3)fucosyltransferases.将人类α-(1,3)岩藻糖基转移酶基因转染至中国仓鼠卵巢细胞。内源性α-(1,3)岩藻糖基转移酶的激活会引发并发症。
J Biol Chem. 1990 Jan 25;265(3):1615-22.
7
LEC12 and LEC29 gain-of-function Chinese hamster ovary mutants reveal mechanisms for regulating VIM-2 antigen synthesis and E-selectin binding.LEC12和LEC29功能获得性中国仓鼠卵巢突变体揭示了调节VIM-2抗原合成和E-选择素结合的机制。
J Biol Chem. 2004 Nov 26;279(48):49716-26. doi: 10.1074/jbc.M408755200. Epub 2004 Sep 13.
8
The LEC11 Chinese hamster ovary mutant synthesizes N-linked carbohydrates containing sialylated, fucosylated lactosamine units. Analysis by one- and two-dimensional 1H NMR spectroscopy.LEC11中国仓鼠卵巢突变体合成含有唾液酸化、岩藻糖基化乳糖胺单元的N-连接碳水化合物。通过一维和二维1H核磁共振光谱分析。
J Biol Chem. 1988 Aug 15;263(23):11374-81.
9
Lectin-resistant variants and revertants of mouse melanoma cells: differential expression of a fucosylated cell-surface antigen and altered metastasizing capacity.
Int J Cancer. 1989 Feb 15;43(2):300-4. doi: 10.1002/ijc.2910430223.
10
The gain-of-function Chinese hamster ovary mutant LEC11B expresses one of two Chinese hamster FUT6 genes due to the loss of a negative regulatory factor.功能获得性中国仓鼠卵巢突变体LEC11B由于一个负调控因子的缺失而表达两个中国仓鼠FUT6基因中的一个。
J Biol Chem. 1999 Apr 9;274(15):10439-50. doi: 10.1074/jbc.274.15.10439.

引用本文的文献

1
Cell-surface enrichment of fucosylated glycoconjugates in the 8- to 16-cell mouse embryo : An autoradiographic study.8至16细胞期小鼠胚胎中岩藻糖基化糖缀合物的细胞表面富集：一项放射自显影研究。
Rouxs Arch Dev Biol. 1987 Dec;196(8):492-498. doi: 10.1007/BF00399873.
2
A Panel of Recombinant Mucins Carrying a Repertoire of Sialylated O-Glycans Based on Different Core Chains for Studies of Glycan Binding Proteins.一组基于不同核心链携带唾液酸化O-聚糖库的重组粘蛋白，用于聚糖结合蛋白的研究。
Biomolecules. 2015 Aug 12;5(3):1810-31. doi: 10.3390/biom5031810.
3
Activation of two new alpha(1,3)fucosyltransferase activities in Chinese hamster ovary cells by 5-azacytidine.
5-氮杂胞苷激活中国仓鼠卵巢细胞中的两种新的α(1,3)岩藻糖基转移酶活性
Cell Regul. 1991 Dec;2(12):989-1000. doi: 10.1091/mbc.2.12.989.
4
Expression of CD15 (FAL) on myeloid cells and chromosomal localization of the gene.髓样细胞上CD15（FAL）的表达及该基因的染色体定位。
Histochem J. 1992 Nov;24(11):777-82. doi: 10.1007/BF01046349.