Mayer V, Valásková M, Gajdosová E, Oravec C
Acta Virol. 1983 May;27(3):228-37.
Dialysates containing transfer factor (TF) activity were prepared from lyzed splenic cells of SPF mice immunized with live, peripherally avirulent Langat virus (TP21 E5 "14" clone) from the tick-borne encephalitis (TBE) complex. The amount of TF was estimated by its capacity to generate in recipient inbred C3H mice cytotoxic T lymphocytes inducing lysis of TBE virus-infected target cells as demonstrated by 51Cr--release assay. A 100 to 1000-fold concentration of TF activity was achieved by combination of the two-step ethanol precipitation of crude dialysates with subsequent fractionation on Sephadex G-25 by exclusion chromatography. Materials from individual concentration steps showed reduced amounts of admixtures, as revealed by absorbance profiles of their chromatograms. In the final product the protein content was most decreased.
用蜱传脑炎（TBE）复合体的活的、外周无毒的兰加特病毒（TP21 E5 “14” 克隆）免疫特定病原体-free（SPF）小鼠，从裂解的脾细胞中制备含有转移因子（TF）活性的透析液。通过其在受体近交C3H小鼠中产生细胞毒性T淋巴细胞的能力来估计TF的量，所述细胞毒性T淋巴细胞可诱导TBE病毒感染的靶细胞裂解，这通过51Cr释放试验得以证明。通过将粗透析液的两步乙醇沉淀与随后在Sephadex G - 25上进行排阻色谱分级相结合，实现了TF活性100至1000倍的浓缩。如各浓度步骤材料的色谱图吸光度谱所示，各浓度步骤的材料显示出减少的杂质含量。最终产品中的蛋白质含量降低最多。
