Papageorgiou N, Carroll M, Durham S R, Lee T H, Walsh G M, Kay A B
Lancet. 1983 Nov 26;2(8361):1220-3. doi: 10.1016/s0140-6736(83)91270-9.
To determine whether neutrophils are activated after exercise-induced asthma, increases in neutrophil complement receptor numbers (complement receptor enhancement) were measured by the rosette technique. In twelve asthmatic patients there was a time-dependent rise in complement receptor numbers for up to 60 min after treadmill exercise. This enhancement of complement receptors was preceded by a rise in plasma neutrophil chemotactic activity and a reduction in the peak expiratory flow rate. These changes could be inhibited by prior administration of disodium cromoglycate. The changes were not observed in seven asthmatic patients in whom asthma was not induced by an identical exercise task. Complement receptor enhancement was also observed in vitro when partially purified neutrophil chemotactic activity from a patient with exercise-induced asthma was incubated with normal neutrophils. These findings suggest that inflammatory cells are activated after exercise-induced asthma, possibly as a result of the release of neutrophil chemotactic activity and other mast-cell-associated mediators.
为了确定运动诱发哮喘后中性粒细胞是否被激活，采用玫瑰花结技术测量了中性粒细胞补体受体数量的增加（补体受体增强）。在12名哮喘患者中，跑步机运动后长达60分钟，补体受体数量呈时间依赖性增加。补体受体的这种增强之前，血浆中性粒细胞趋化活性升高，呼气峰值流速降低。这些变化可被预先给予色甘酸钠抑制。在7名未通过相同运动任务诱发哮喘的哮喘患者中未观察到这些变化。当将运动诱发哮喘患者的部分纯化中性粒细胞趋化活性与正常中性粒细胞一起孵育时，在体外也观察到了补体受体增强。这些发现表明，运动诱发哮喘后炎症细胞被激活，可能是中性粒细胞趋化活性和其他肥大细胞相关介质释放的结果。
