Porter L A, Rawls J M
Mol Gen Genet. 1984;193(1):27-32. doi: 10.1007/BF00327409.
Mutations at the Dhod locus have been isolated following ethylmethanesulfonate mutagenesis. These mutants express those phenotypes common to other mutations of the de novo pyrimidine pathway: specific wing and leg defects and female sterility. Dihydroorotate dehydrogenase activity is severely reduced in all Dhod mutants, whereas levels of the other pathway enzymes are largely unaffected. The twelve Dhod mutations described here comprise a single complementation group. All of these mutations are nonlethal and the collection includes apparent amorphic as well as hypomorphic alleles. These results are discussed relative to the properties of the complex loci that encode the other steps of de novo pyrimidine biosynthesis.
在甲磺酸乙酯诱变后分离出了Dhod位点的突变。这些突变体表现出与从头嘧啶途径其他突变共有的那些表型：特定的翅膀和腿部缺陷以及雌性不育。在所有Dhod突变体中，二氢乳清酸脱氢酶活性严重降低，而其他途径酶的水平基本未受影响。这里描述的12个Dhod突变组成一个单一的互补群。所有这些突变都是非致死的，并且该集合包括明显的无效等位基因以及亚效等位基因。相对于编码从头嘧啶生物合成其他步骤的复杂位点的特性，对这些结果进行了讨论。
