Jones W K, Rawls J M
T.H. Morgan School of Biological Sciences, University of Kentucky, Lexington 40506.
Genetics. 1988 Nov;120(3):733-42. doi: 10.1093/genetics/120.3.733.
Chromosome region 85A contains at least 12 genetic complementation groups, including the genes dhod, pink and hunchback. In order to better understand the organization of this chromosomal segment and to permit molecular studies of these genes, we have carried out a genetic analysis coupled with a chromosome walk to isolate the DNA containing these genes. Complementation tests with chromosomal deficiencies permitted unambiguous ordering of most of the complementation groups identified within the 85A region. Recombinant bacteriophage clones were isolated that collectively span over 120 kb of 85A DNA and these were used to produce a molecular map of the region. The breakpoint sites of a number of 85A chromosome rearrangements were localized on the molecular map, thereby delimiting regions of the DNA that contain the various genetic complementation groups.
染色体区域85A包含至少12个遗传互补群，其中包括基因dhod、pink和驼背基因。为了更好地理解该染色体片段的组织结构，并对这些基因进行分子研究，我们进行了遗传分析并结合染色体步移来分离包含这些基因的DNA。利用染色体缺失进行的互补测试使得能够明确排列85A区域内鉴定出的大多数互补群。分离出了总共跨越超过120 kb的85A DNA的重组噬菌体克隆，并利用这些克隆制作了该区域的分子图谱。许多85A染色体重排的断点位点定位在了分子图谱上，从而界定了包含各种遗传互补群的DNA区域。
