Bhathena S J, Awoke S, Voyles N R, Wilkins S D, Recant L, Oie H K, Gazdar A F
Proc Soc Exp Biol Med. 1984 Jan;175(1):35-8. doi: 10.3181/00379727-175-41762.
Cells derived from rat islet tumor and grown in culture (parent cells-RIN-m) and two clones obtained from them were used to study the effect of various secretagogues on insulin, glucagon, and somatostatin secretion. Parent cells secreted all three hormones in various quantities, while clone 5F secreted predominantly insulin and clone 14B secreted predominantly somatostatin. The secretory behavior of these cells were compared to each other and to that of normal islets. In general, as in the case of normal islets, insulin secretion was stimulated by calcium, potassium, tolbutamide, theophylline, and glucagon. It was inhibited by somatostatin. Glucagon secretion was stimulated by calcium, arginine, and theophylline. Somatostatin secretion was stimulated in clone 14B by arginine, tolbutamide, theophylline, and insulin. These cells differ from normal islets, in that they do not respond to glucose or arginine with increased insulin secretion. Also somatostatin failed to inhibit glucagon secretion. The similarity in insulin secretory responses of parent cells and clone 5F suggests that local or paracrine islet hormone secretion plays only a negligible role in the control of other hormone secretion in these cells.
源自大鼠胰岛肿瘤并在培养物中生长的细胞（亲本细胞 - RIN - m）以及从它们获得的两个克隆用于研究各种促分泌素对胰岛素、胰高血糖素和生长抑素分泌的影响。亲本细胞分泌这三种激素的量各不相同，而克隆5F主要分泌胰岛素，克隆14B主要分泌生长抑素。将这些细胞的分泌行为相互比较，并与正常胰岛的分泌行为进行比较。一般来说，与正常胰岛的情况一样，胰岛素分泌受到钙、钾、甲苯磺丁脲、茶碱和胰高血糖素的刺激。它受到生长抑素的抑制。胰高血糖素分泌受到钙、精氨酸和茶碱的刺激。在克隆14B中，精氨酸、甲苯磺丁脲、茶碱和胰岛素刺激生长抑素分泌。这些细胞与正常胰岛不同，因为它们对葡萄糖或精氨酸没有胰岛素分泌增加的反应。而且生长抑素未能抑制胰高血糖素分泌。亲本细胞和克隆5F胰岛素分泌反应的相似性表明，局部或旁分泌胰岛激素分泌在这些细胞中对其他激素分泌的控制中仅起微不足道的作用。
