Zaleski M B, Reichner J S, Albini B
Int Arch Allergy Appl Immunol. 1984;73(3):263-8. doi: 10.1159/000233479.
Primary and secondary anti-Thy-1 responses were elicited in C57BL/6Kh and (C57BL/6Kh X BALB/cKh)F1 mice by injection of either intact or sonicated Thy-1 disparate thymocytes as the source of the two forms of Thy-1 antigen--cell bound and cell free, respectively. The primary response was characterized by a high number of anti-Thy-1 plaque-forming cells in the spleen and a high titer of serum antibodies that belonged predominantly to the IgM class. The secondary response consisted of a moderate number of plaque-forming cells and a high titer of serum antibodies that belonged to the IgG and IgM classes. Only secondary responses ensued in mice primed and boosted with the same form of antigen, whereas primary and secondary responses developed concomitantly in animals primed with one form and boosted with both forms simultaneously. These data suggested that the two forms of Thy-1 antigen activate in a given responder two discrete subsets of T cells.
通过注射完整的或经超声处理的Thy-1不同胸腺细胞，分别作为两种形式的Thy-1抗原（细胞结合型和游离型）的来源，在C57BL/6Kh和（C57BL/6Kh×BALB/cKh）F1小鼠中引发了初次和二次抗Thy-1反应。初次反应的特征是脾脏中有大量抗Thy-1斑块形成细胞，血清抗体滴度高，且主要属于IgM类。二次反应由中等数量的斑块形成细胞和属于IgG和IgM类的高滴度血清抗体组成。只用同一种形式的抗原进行致敏和加强免疫的小鼠只出现二次反应，而用一种形式的抗原进行致敏并同时用两种形式的抗原进行加强免疫的动物则同时出现初次和二次反应。这些数据表明，两种形式的Thy-1抗原在给定的应答者中激活了两个离散的T细胞亚群。
