Glimcher M J
Philos Trans R Soc Lond B Biol Sci. 1984 Feb 13;304(1121):479-508. doi: 10.1098/rstb.1984.0041.
The most widely accepted hypothesis to account for maturational changes in the X-ray diffraction characteristics of bone mineral has been the 'amorphous calcium phosphate theory', which postulates that an initial amorphous calcium phosphate solid phase is deposited that gradually converts to poorly crystalline hydroxyapatite. Our studies of bone mineral of different ages by X-ray radial distribution function analysis and 31P n.m.r. have conclusively demonstrated that a solid phase of amorphous calcium phosphate does not exist in bone in any significant amount. 31P n.m.r. studies have detected the presence of acid phosphate groups in a brushite-like configuration. Phosphoproteins containing O-phosphoserine and O-phosphothreonine have been isolated from bone matrix and characterized. Tissue and cell culture have established that they are synthesized in bone, most likely by the osteoblasts. Physiochemical and pathophysiological studies support the thesis that the mineral and organic phases of bone and other vertebrate mineralized tissues are linked by the phosphomonester bonds of O-phosphoserine and O-phosphothreonine, which are constituents of both the structural organic matrix and the inorganic calcium phosphate crystals.
关于骨矿物质X射线衍射特征成熟变化的最广泛接受的假说是“无定形磷酸钙理论”，该理论假定最初沉积的是无定形磷酸钙固相，其逐渐转化为结晶度差的羟基磷灰石。我们通过X射线径向分布函数分析和31P核磁共振对不同年龄骨矿物质的研究已确凿证明，骨中不存在任何大量的无定形磷酸钙固相。31P核磁共振研究已检测到以透钙磷石样构型存在的酸性磷酸基团。含有O-磷酸丝氨酸和O-磷酸苏氨酸的磷蛋白已从骨基质中分离并进行了表征。组织和细胞培养已证实它们在骨中合成，很可能由成骨细胞合成。物理化学和病理生理学研究支持这样的论点，即骨和其他脊椎动物矿化组织的矿物质和有机相通过O-磷酸丝氨酸和O-磷酸苏氨酸的磷酸单酯键相连，这两者都是结构有机基质和无机磷酸钙晶体的组成部分。
