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[垂体在高渗溶液中孵育后腺垂体细胞的亚显微研究。I. 酸性磷酸酶、硫胺素焦磷酸酶和芳基硫酸酯酶活性的分布]

[Submicroscopic study of adenohypophyseal cells with the hypophysis incubated in a hypertonic solution. I. The distribution of acid phosphatase, thiamine pyrophosphatase and arylsulfatase activities].

作者信息

Nevorotin A I, Khokhlov S E

出版信息

Tsitologiia. 1983 Dec;25(12):1361-5.

PMID:6142552
Abstract

In the rat adenohypophyseal mammotrophs and somatotrophs, activities of acid phosphatase, thiamine pyrophosphatase and aryl sulphatase were defined after the incubation of the pituitary gland in the medium containing 1.65 M sucrose. Following 60--120 minutes of the incubation the above enzymes were found, in addition to their usual sites, in all cisternae of the Golgi apparatus and the rough endoplasmic reticulum as well as within the perinuclear cisternae. The mechanism of the above alterations is discussed, and a suggestion is put forward that the emergence of enzymatic activities in the above structures may reflect their biogenetic pathways in the cell.

摘要

在大鼠腺垂体的促乳腺激素细胞和促生长激素细胞中,将垂体在含有1.65M蔗糖的培养基中孵育后,测定了酸性磷酸酶、硫胺素焦磷酸酶和芳基硫酸酯酶的活性。孵育60至120分钟后,除了这些酶通常所在的部位外,在高尔基体和糙面内质网的所有潴泡以及核周潴泡中都发现了它们。讨论了上述变化的机制,并提出上述结构中酶活性的出现可能反映了它们在细胞中的生物发生途径。

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