Dambinova S A, Gorodinskiĭ A I
Biokhimiia. 1984 Jan;49(1):67-74.
The binding of L-[3H]glutamate to rat cerebral cortex synaptic membranes was investigated. Two types of binding sites, a Na+-independent (Kd = 140-160 nm; Bmax = 3.8-4.5 pmol-mg of protein) and a Na+-dependent (Kd = 2.0 microM; Bmax = 45-50 pmol/mg of protein) ones, were detected. The dependence of Na+-insensitive binding on time and temperature and membrane content in a sample was determined. Mono- and divalent cations (5-10 mM) potentiated specific binding by 2.1-3.3 times. The Na+-dependent binding is associated with active transport systems, while the Na+-independent one-with true receptor binding. The relationship between CNS glutamate receptors and Na+-independent binding sites is discussed.
研究了L-[3H]谷氨酸与大鼠大脑皮质突触膜的结合。检测到两种结合位点，一种是不依赖钠离子的（解离常数Kd = 140 - 160 nM；最大结合容量Bmax = 3.8 - 4.5 pmol/mg蛋白质），另一种是依赖钠离子的（Kd = 2.0 μM；Bmax = 45 - 50 pmol/mg蛋白质）。测定了不依赖钠离子的结合对时间、温度以及样品中膜含量的依赖性。单价和二价阳离子（5 - 10 mM）使特异性结合增强2.1 - 3.3倍。依赖钠离子的结合与主动转运系统有关，而不依赖钠离子的结合与真正的受体结合有关。讨论了中枢神经系统谷氨酸受体与不依赖钠离子的结合位点之间的关系。
