Kozik M B, Szczech J
Exp Pathol. 1983;24(4):243-52. doi: 10.1016/s0232-1513(83)80012-7.
The effect of treatment with vincristine on nerve cells of gyrus cinguli was studied. Wistar rats aged 1, 3, 7, 14, 21 and 28 days were given single intraperitoneal injections of vincristine (0.01 mg/kg body weight). All experimental animals were decapitated at the age of 29 days and their brains subjected to histoenzymatic and morphometric examination. The control group consisted of untreated rats aged 29 days. The activity of phosphatases and esterases of the cingulum was evaluated histochemically and the morphometric parameters of pyramidal cells in this region were determined by means of a computer microscope "Morphoquant" (VEB Carl Zeiss JENA). The results of this study have shown that administration of therapeutic doses of vincristine to young rats brings about a drop of the neuronal AChE and NsE activity, contrary, to this, TPPase and acP activities of the pyramidal cells are enhanced by this treatment. Morphometric determinations have revealed elongation of the neuronal perikaryons along with an increase of indices of the cytoplasmatic area and volume of pyramidal cells. The observed alterations are considered to reflect degenerative processes of moderate intensity.
研究了长春新碱治疗对扣带回神经细胞的影响。给1、3、7、14、21和28日龄的Wistar大鼠单次腹腔注射长春新碱（0.01mg/kg体重）。所有实验动物在29日龄时断头，其大脑进行组织酶学和形态计量学检查。对照组由29日龄未治疗的大鼠组成。通过组织化学方法评估扣带的磷酸酶和酯酶活性，并借助计算机显微镜“Morphoquant”（VEB Carl Zeiss JENA）测定该区域锥体细胞的形态计量参数。本研究结果表明，给幼鼠施用治疗剂量的长春新碱会导致神经元乙酰胆碱酯酶和神经特异性酯酶活性下降，与此相反，这种治疗会增强锥体细胞的三磷酸腺苷酶和酸性磷酸酶活性。形态计量学测定显示神经元胞体延长，同时锥体细胞的细胞质面积和体积指数增加。观察到的改变被认为反映了中等强度的退行性过程。
