Kubilus J, Baden H P
Mol Cell Biochem. 1984;58(1-2):129-37. doi: 10.1007/BF00240612.
Proteins which are major substrates of epidermal transglutaminases can be identified in cultured keratinocytes of human, cow, and new-born rat. Cow and human keratinocytes both contain substrate proteins which are 30 000 to 50 000 daltons in size but dissociable in SDS to 12 000 daltons or less. In both species these proteins correspond to in vivo synthesized proteins which are probable precursors of cornified envelope. Human keratinocytes synthesize a 125 000 dalton protein which is also a precursor of cornified envelope both in cells and tissue. By SDS electrophoresis two 100 000 dalton substrate proteins are seen in cow keratinocyte extracts and a 23 000 dalton substrate protein is seen in rat keratinocyte extracts. Minor substrates of transglutaminase are seen in human keratinocytes, and one has been isolated by preparative electrophoresis. Major structural proteins of epidermis which are in vitro substrates of epidermal transglutaminase include the keratins and the stratum corneum basic protein.
在人、牛和新生大鼠的培养角质形成细胞中，可以鉴定出作为表皮转谷氨酰胺酶主要底物的蛋白质。牛和人的角质形成细胞都含有大小在30000至50000道尔顿之间但在SDS中可解离为12000道尔顿或更小的底物蛋白。在这两个物种中，这些蛋白质对应于体内合成的蛋白质，它们可能是角质包膜的前体。人角质形成细胞合成一种125000道尔顿的蛋白质，它在细胞和组织中也是角质包膜的前体。通过SDS电泳，在牛角质形成细胞提取物中可见两种100000道尔顿的底物蛋白，在大鼠角质形成细胞提取物中可见一种23000道尔顿的底物蛋白。在人角质形成细胞中可见转谷氨酰胺酶的次要底物，其中一种已通过制备电泳分离出来。作为表皮转谷氨酰胺酶体外底物的表皮主要结构蛋白包括角蛋白和角质层碱性蛋白。
