Brown R H, Schweitzer J, Dichter M A
Brain Res. 1984 Mar 26;296(1):87-91. doi: 10.1016/0006-8993(84)90513-4.
Immunofluorescent studies of the Thy-1 antigen in cultures of mouse spinal cord demonstrated positivity on the surfaces of a fraction of neurons and glia. Positivity was evident by one week and increased slowly over the lifespan of the cultures, often several months. Thy-1 positivity was most apparent in regions of clustering of neurons and glia. Neuroglial clusters were identified by morphologic appearance; the identity of astrocytes was confirmed by immunofluorescent staining of glial fibrillary acidic protein.
对小鼠脊髓培养物中Thy-1抗原的免疫荧光研究表明,一部分神经元和神经胶质细胞表面呈阳性。一周时阳性明显,在培养物的整个生命周期(通常为几个月)中缓慢增加。Thy-1阳性在神经元和神经胶质细胞聚集区域最为明显。通过形态学外观识别神经胶质细胞簇;通过胶质纤维酸性蛋白的免疫荧光染色确认星形胶质细胞的身份。