Blinova N N, Frolov I V, Beklemishev A B, Mertvetsov N P
Biokhimiia. 1984 Mar;49(3):470-8.
The effects of the glucocorticoid hydrocortisone on the synthesis of specific template RNA coding tyrosine aminotransferase (TAT) in rat liver during hormonal induction were studied. Using hybridization of complementary DNA (cDNA-TAT) with polysomal liver poly-A-mRNA, the content of specific mRNA-TAT in liver polysomes during and after hormonal induction, i. e. 4 and 16 hrs after hydrocortisone injection, respectively, was estimated. It was shown that 4 hrs after the hormone injection that mRNA-TAT content in liver polysomes is increased 3-4-fold, showing a return to the initial level after 16 hrs. Thus, transcription is the main link in the realization of glucocorticoid induction.
研究了糖皮质激素氢化可的松在激素诱导过程中对大鼠肝脏中编码酪氨酸转氨酶（TAT）的特定模板RNA合成的影响。利用互补DNA（cDNA-TAT）与肝脏多聚核糖体多聚腺苷酸mRNA杂交，分别估算了激素诱导期间及诱导后，即氢化可的松注射后4小时和16小时，肝脏多聚核糖体中特定mRNA-TAT的含量。结果表明，激素注射后4小时，肝脏多聚核糖体中mRNA-TAT的含量增加了3至4倍，16小时后又恢复到初始水平。因此，转录是糖皮质激素诱导作用实现的主要环节。
