在蟹（黄道蟹）金属硫蛋白于二乙氨基乙基纤维素上进行色谱分离期间使用2-巯基乙醇。

Use of 2-mercaptoethanol during chromatography of crab (Cancer pagurus) metallothionein on DEAE cellulose.

作者信息

Overnell J

出版信息

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;77(2):245-8. doi: 10.1016/0742-8413(84)90008-2.

Abstract

The chromatographic properties of crab metallothionein appear particularly sensitive to oxidation, and this oxidation does not take place when 2-mercaptoethanol is present in the buffers. The chromatographic changes brought about by oxidation can be reversed by subsequent addition of 2-mercaptoethanol.

摘要

蟹金属硫蛋白的色谱特性似乎对氧化特别敏感，当缓冲液中存在2-巯基乙醇时，这种氧化就不会发生。氧化引起的色谱变化可通过随后添加2-巯基乙醇来逆转。