Olcese J M, Hall T R, Figueroa H R, deVlaming V L
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;77(2):335-7. doi: 10.1016/0742-8413(84)90024-0.
The antiestrogen, clomiphene citrate ( Merrell -National Laboratories) was administered to female goldfish in order to test the hypothesis that this drug may act on brain monoaminergic mechanisms. Brain monoamine oxidase (MAO) activity and hypothalamic serotonin (5-HT) content were measured fluorometrically after i.p. administration of 0, 5 or 25 micrograms clomiphene citrate/g body wt. Brain MAO activity was significantly inhibited by the high dose of clomiphene citrate, whereas hypothalamic 5-HT content was significantly elevated by both low and high doses of the antiestrogen. These data support the idea that brain monoamines in teleost fish are influenced by estrogen feedback mechanisms which can be blocked by clomiphene citrate.
为了验证克罗米酚柠檬酸盐（美里尔国家实验室生产）这种抗雌激素药物可能作用于脑单胺能机制的假说，对雌性金鱼施用了该药物。腹腔注射0、5或25微克/克体重的克罗米酚柠檬酸盐后，用荧光法测定脑单胺氧化酶（MAO）活性和下丘脑5-羟色胺（5-HT）含量。高剂量的克罗米酚柠檬酸盐可显著抑制脑MAO活性，而低剂量和高剂量的抗雌激素均可显著提高下丘脑5-HT含量。这些数据支持了这样一种观点，即硬骨鱼脑中的单胺受雌激素反馈机制影响，而克罗米酚柠檬酸盐可阻断该机制。
