Janssens P A, Grigg J A
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;77(2):403-8. doi: 10.1016/0742-8413(84)90035-5.
Glycogenolysis was stimulated by catecholamines in in vitro cultures of hepatic tissue of Xenopus laevis. Dose response curves showed that adrenaline and isoprenaline were equally effective while noradrenaline and phenylephrine were progressively less effective in eliciting glycogen breakdown. Neither oxymetazoline nor methoxamine had any effect on glycogenolysis. Administration of adrenaline to cultures was followed within 1 min by a rise in tissue cyclic AMP concentration and within 2 min by an increase in phosphorylase a activity. Both these responses were blocked by propranolol but little affected by phenoxybenzamine. These findings suggest that catecholamines activate glycogenolysis via a beta-adrenergic receptor in X. laevis and that alpha-adrenergic receptors play no role in regulating hepatic glycogenolysis in this species.
在非洲爪蟾肝组织的体外培养中，儿茶酚胺刺激糖原分解。剂量反应曲线表明，肾上腺素和异丙肾上腺素在引发糖原分解方面效果相同，而去甲肾上腺素和苯肾上腺素引发糖原分解的效果则逐渐减弱。羟甲唑啉和甲氧明对糖原分解均无任何作用。向培养物中加入肾上腺素后，组织环磷酸腺苷（cAMP）浓度在1分钟内升高，磷酸化酶a活性在2分钟内增加。这两种反应均被普萘洛尔阻断，但很少受酚苄明影响。这些发现表明，儿茶酚胺通过非洲爪蟾的β-肾上腺素能受体激活糖原分解，且α-肾上腺素能受体在该物种肝糖原分解的调节中不起作用。
