Synaptic contacts of somatostatin-immunoreactive amacrine cells in goldfish retina.

Retinal amacrine cells containing somatostatinlike immunoreactivity (SLI) were labeled by the peroxidase-antiperoxidase technique and their connections were analyzed by light and electron microscopy. The labeled processes were found in two distinct plexuses--one near the most proximal border of the inner plexiform layer and the other near the most distal border. They received most (89%) of their input from amacrine cells and the remainder from bipolar cells. A majority (56%) of their output synapses go to processes of amacrine cells, a substantial proportion (38%) go to ganglion cell dendrites, and the remainder go to bipolar cell axon terminals. The relative frequencies of each of the types of contacts were nearly identical in the distal and proximal plexuses. The cells containing SLI are different in their morphology and synaptic connections from any goldfish amacrine cells containing conventional neurotransmitters, but one type of amacrine cell containing SLI resembles certain other peptidergic amacrine cells in the goldfish retina.