Harm W, Deamer D W
Physiol Chem Phys. 1977;9(6):501-12.
Erythrocytes of vitamin E-deficient rats were investigated as an in vivo model of oxidant stress and cellular aging. To measure possible membrane damage related to the enhanced oxidant stress, the permeability of the erythrocyte membrane to potassium was determined. Rates of non-hemolytic potassium loss were calculated from comparison of total potassium loss and hemolysis rates. The non-hemolytic potassium loss rates for erythrocytes of vitamin E-deficient rats were as much as 2.5-fold higher than controls. The abnormally high permeability of vitamin E-deficient rat erythrocytes indicates molecular damage at the membrane level, and may be significant to our understanding of the normal aging process in erythrocytes and other cells.
将维生素E缺乏的大鼠红细胞作为氧化应激和细胞衰老的体内模型进行研究。为了测量与增强的氧化应激相关的可能的膜损伤，测定了红细胞膜对钾的通透性。通过比较总钾损失和溶血率来计算非溶血钾损失率。维生素E缺乏的大鼠红细胞的非溶血钾损失率比对照组高2.5倍。维生素E缺乏的大鼠红细胞异常高的通透性表明膜水平存在分子损伤，这可能对我们理解红细胞和其他细胞的正常衰老过程具有重要意义。
