Helwig J J, Judes C, Bollack C, Mandel P
Ren Physiol. 1984 May-Jun;7(3):146-55. doi: 10.1159/000172933.
Collagenasic dispersion of rabbit kidney cortex followed by centrifugation on discontinuous sucrose gradient, allowed the simultaneous isolation of a microvascular fraction and of glomerular and tubular fractions. The microvessels were characterized by an overall diameter of 22 micron and the presence of granular and smooth muscle cells. Measurement of cellular renin activity and the muscle specific enzyme creatine kinase showed that these vessels were arteriolar in nature and that they contained the preglomerular arterioles. The glomerular or tubular contamination rates were assessed by means of enzymatic markers. Thus, in the arterioles, potassium fluoride-resistant acid phosphatase was some 10 times lower than in the glomeruli. The specific tubular enzymes gamma-glutamyl-transpeptidase and alkaline phosphatase were about 20 times lower in the arterioles than in the tubular fraction. The possibility of obtaining these fractions may facilitate many types of study on renal hemodynamic and glomerulo-tubular feedback control.
对兔肾皮质进行胶原酶消化，然后在不连续蔗糖梯度上离心，可同时分离出微血管部分以及肾小球和肾小管部分。微血管的特征是总体直径为22微米，且存在颗粒细胞和平滑肌细胞。对细胞肾素活性和肌肉特异性酶肌酸激酶的测量表明，这些血管本质上是小动脉，并且包含入球小动脉。通过酶标记物评估肾小球或肾小管的污染率。因此，在小动脉中，耐氟化钾酸性磷酸酶比在肾小球中低约10倍。小动脉中的特异性肾小管酶γ-谷氨酰转肽酶和碱性磷酸酶比肾小管部分低约20倍。获得这些部分的可能性可能有助于对肾血流动力学和肾小球-肾小管反馈控制进行多种类型的研究。
