Sarafian T A, Fluharty A L, Kihara H
Biochem Med. 1984 Feb;31(1):80-6. doi: 10.1016/0006-2944(84)90062-0.
Cerebroside sulfatase also known as arylsulfatase A from human liver displays six microheteromer bands upon narrow pH range isoelectric focusing. Sialic acid residues only partially account for this enzyme multiplicity since neuraminidase treatment reduces the number of bands to three. Uptake studies with cultured fibroblasts strongly suggest arylsulfatase A has covalently bound mannose 6-phosphate residues. However, treatment with alkaline phosphatase and a battery of glycohydrolases failed to reduce the number of enzyme charge forms below three. These results imply that the neuraminidase-resistant charge microheterogeneity is not due to structures associated with the carbohydrate moiety of arylsulfatase A.
脑苷脂硫酸酯酶也称为来自人肝脏的芳基硫酸酯酶A，在窄pH范围等电聚焦时显示出六条微异聚体带。唾液酸残基仅部分解释了这种酶的多样性，因为神经氨酸酶处理可将带数减少至三条。对培养的成纤维细胞的摄取研究强烈表明芳基硫酸酯酶A具有共价结合的甘露糖6-磷酸残基。然而，用碱性磷酸酶和一系列糖水解酶处理未能将酶电荷形式的数量减少到三条以下。这些结果表明，抗神经氨酸酶的电荷微异质性不是由于与芳基硫酸酯酶A的碳水化合物部分相关的结构所致。
