Grady R K, Caliguri A, Mefford I N
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;78(1):141-3. doi: 10.1016/0742-8413(84)90061-6.
Day/night differences in concentrations of 5-hydroxy and 5-methoxy indole metabolites in the pineal gland of the pigeon are described. A simultaneous determination of 5-hydroxytryptamine (serotonin), 5-hydroxyindoleacetic acid, 5-hydroxytryptophol, N-acetyl-5-hydroxytryptamine (N-acetyl serotonin), 5-methoxyindoleacetic acid, 5-methoxytryptophol, tryptophan, indoleacetic acid and melatonin was accomplished using a recently developed procedure employing high-performance liquid chromatography with electrochemical detection. As in mammalian species, an inverse relationship was observed between N-acetylated indoles and serotonin and its acid metabolites. Melatonin and N-acetyl serotonin were increased approximately three-fold at night to concentrations of 0.730 and 1.79 ng/pineal respectively. Daytime serotonin values were 44.9 +/- 13.0 ng/pineal and decreased to 12.3 +/- 6.5 ng/pineal during the dark phase.
本文描述了鸽子松果体中5-羟基和5-甲氧基吲哚代谢物浓度的昼夜差异。采用最近开发的高效液相色谱-电化学检测方法，同时测定了5-羟色胺（血清素）、5-羟基吲哚乙酸、5-羟基色醇、N-乙酰-5-羟色胺（N-乙酰血清素）、5-甲氧基吲哚乙酸、5-甲氧基色醇、色氨酸、吲哚乙酸和褪黑素。与哺乳动物一样，观察到N-乙酰化吲哚与血清素及其酸性代谢物之间呈负相关。褪黑素和N-乙酰血清素在夜间分别增加到约三倍，浓度分别为0.730和1.79 ng/松果体。白天血清素值为44.9±13.0 ng/松果体，在黑暗阶段降至12.3±6.5 ng/松果体。
