胚胎年龄对鸡和鹌鹑胚胎神经视网膜聚集性、组织发生及生化分化的影响。

Effect of embryonic age on aggregability, histogenesis and biochemical differentiation in the embryonic chick and quail neural retina.

作者信息

Li G X, Moscona M H, Moscona A A

出版信息

Sci Sin B. 1984 Apr;27(4):371-9.

Abstract

In this paper, the different ages of the chicken and quail embryonic retina cell aggregates are used to examine the aggregation, histogenesis, and the induction of glutamine synthetase (GS) elicited by hydrocortisone. The results show that the GS inducibility in the retina cells increases at a slow rate, then rises sharply on the 9th day, and after 9 days, the GS inducibility of aggregates decreases with the increase of embryonic age. The observed value of induced GS activity in the chick retina cells coaggregate with quail embryonic retina cells is less than the expected value. Bispecific cell coaggregates were observed when the 9-day chicken embryonic retina cells and the 71/2-day quail embryonic retina cells were combined and coaggregated, the chicken and quail cells formed three retinal rosettes. When the 9-day chicken embryonic retina cells were commingled with the 13-day quail embryonic retina cells, in the coaggregates, only one retinal rosette was formed.

摘要

在本文中，利用不同年龄的鸡和鹌鹑胚胎视网膜细胞聚集体来检测氢化可的松引发的细胞聚集、组织发生以及谷氨酰胺合成酶（GS）的诱导情况。结果表明，视网膜细胞中GS的诱导能力起初缓慢增加，在第9天急剧上升，9天后，聚集体的GS诱导能力随着胚胎年龄的增加而下降。鸡视网膜细胞与鹌鹑胚胎视网膜细胞共聚集时，诱导的GS活性观测值低于预期值。当将9日龄鸡胚胎视网膜细胞与7.5日龄鹌鹑胚胎视网膜细胞组合并共聚集时，观察到双特异性细胞共聚集体，鸡和鹌鹑细胞形成了三个视网膜玫瑰花结。当9日龄鸡胚胎视网膜细胞与13日龄鹌鹑胚胎视网膜细胞混合时，在共聚集体中仅形成了一个视网膜玫瑰花结。