Jung K, Bader K, Grützmann K D
Enzyme. 1984;31(4):209-16. doi: 10.1159/000469528.
We analyzed the stability of the enzymes alpha-amylase (EC 3.2.1.1), alkaline phosphatase (EC 3.1.3.1), alanine aminotransferase (EC 2.6.1.2), aspartate aminotransferase (EC 2.6.1.1), creatine kinase (EC 2.7.3.2), glutamate dehydrogenase (EC 1.4.1.3), gamma-glutamyltransferase (EC 2.3.2.2) and lactate dehydrogenase (EC 1.1.1.27) of a human serum pool during storage in liquid nitrogen for a period of 10 months. Except amylase and creatine kinase, all enzymes were stable. Amylase increased in activity, creatine kinase activity decreased. Therefore, human serum stored at -196 degrees C can be used as satisfactory substitute for lyophilized enzyme control serum in internal quality control and stable enzyme material for optimization of methods.
我们分析了人血清库中的α-淀粉酶（EC 3.2.1.1）、碱性磷酸酶（EC 3.1.3.1）、丙氨酸转氨酶（EC 2.6.1.2）、天冬氨酸转氨酶（EC 2.6.1.1）、肌酸激酶（EC 2.7.3.2）、谷氨酸脱氢酶（EC 1.4.1.3）、γ-谷氨酰转移酶（EC 2.3.2.2）和乳酸脱氢酶（EC 1.1.1.27）在液氮中储存10个月期间的稳定性。除淀粉酶和肌酸激酶外，所有酶均稳定。淀粉酶活性增加，肌酸激酶活性降低。因此，储存在-196℃的人血清可作为内部质量控制中冻干酶对照血清的满意替代品，以及用于方法优化的稳定酶材料。
