Wigham T, Batten T F
Gen Comp Endocrinol. 1984 Sep;55(3):444-9. doi: 10.1016/0016-6480(84)90016-9.
Pituitary glands were removed from Poecilia latipinna which had been maintained in one-third seawater and were incubated for 18 hr in media of either 300 mosmol/kg (OP300) or 340 mosmol/kg (OP340) osmotic pressure for measurement of both total and newly synthesised prolactin (PRL) and growth hormone (GH) release. Thyrotropin-releasing hormone (TRH) at 100 ng/ml increased release of total and newly synthesised PRL into OP340, but not into OP300, medium. Conversely, 300 ng/ml of somatotropin-release-inhibiting factor (SRIF) inhibited total and newly synthesised PRL release into OP300, but not OP340, medium. At 1000 ng/ml, SRIF inhibited total PRL release into both media, but newly synthesised PRL release was reduced significantly only in OP300 medium. The release of GH was unaffected by 100 ng/ml TRH in OP300 medium, but both total and newly synthesised GH release were enhanced by this dose in OP340 medium. SRIF at 300 ng/ml reduced total GH release into OP300 medium, whereas the release of newly synthesised GH was inhibited in OP340 medium. At 1000 ng/ml, SRIF inhibited total GH release into both media, but release of the newly synthesised hormone was not significantly altered. These results suggest that TRH can stimulate and SRIF inhibit both PRL and GH release by Poecilia pituitaries, but that these effects may be modulated by plasma osmotic pressure.
从饲养在三分之一海水环境中的新月鱼身上取出脑垂体，将其在渗透压为300毫渗摩尔/千克（OP300）或340毫渗摩尔/千克（OP340）的培养基中孵育18小时，以测量总催乳素（PRL）和新合成催乳素以及生长激素（GH）的释放情况。100纳克/毫升的促甲状腺激素释放激素（TRH）可增加OP340培养基中总催乳素和新合成催乳素的释放，但在OP300培养基中则无此作用。相反，300纳克/毫升的生长抑素释放抑制因子（SRIF）可抑制OP300培养基中总催乳素和新合成催乳素的释放，但对OP340培养基无此作用。在1000纳克/毫升时，SRIF可抑制两种培养基中总催乳素的释放，但仅在OP300培养基中新合成催乳素的释放显著减少。OP300培养基中100纳克/毫升的TRH对GH的释放无影响，但在OP340培养基中该剂量可增强总GH和新合成GH的释放。300纳克/毫升的SRIF可减少OP300培养基中总GH的释放，而在OP340培养基中新合成GH的释放受到抑制。在1000纳克/毫升时，SRIF可抑制两种培养基中总GH的释放，但新合成激素的释放无显著变化。这些结果表明，TRH可刺激新月鱼脑垂体释放PRL和GH，SRIF则可抑制其释放，但这些作用可能受血浆渗透压的调节。
