Benoit R, Böhlen P, Esch F, Ling N
Brain Res. 1984 Oct 8;311(1):23-9. doi: 10.1016/0006-8993(84)91394-5.
Two neuropeptides reacting with antibodies directed against the C-terminal region of somatostatin 28(1-12) were purified to homogeneity from rat brain extracts. Amino acid analysis revealed that the larger peptide (8 kdaltons) consisted of 76 amino acids. Microsequencing established its aminoterminal structure as: Ala-Pro-Ser-Asp-Pro-Arg-Leu-Arg-Gln-Phe-X-Gln-Lys. The 8 kdalton somatostatin 28(1-12)-like peptide corresponds to the whole prosomatostatin molecule without Arg-Lys-somatostatin-14. The smaller peptide (5 kdaltons) consists of 44 amino acids and is generated after cleavage of a Leu-Leu bond at position 56-57 of pre-prosomatostatin. Both 8 kdalton and 5 kdalton somatostatin 28(1-12)-like peptides contain somatostatin 28(1-12) at their C-termini. The 4 most abundant neuropeptides derived from pre-prosomatostatin (pre-proSS) and presently characterized are: somatostatin-14, somatostatin 28(1-12), somatostatin 28 and pre-proSS25-100.
从大鼠脑提取物中纯化出两种与抗生长抑素28(1 - 12) C末端区域抗体发生反应的神经肽，使其达到同质。氨基酸分析表明，较大的肽（8千道尔顿）由76个氨基酸组成。微量测序确定其氨基末端结构为：丙氨酸 - 脯氨酸 - 丝氨酸 - 天冬氨酸 - 脯氨酸 - 精氨酸 - 亮氨酸 - 精氨酸 - 谷氨酰胺 - 苯丙氨酸 - X - 谷氨酰胺 - 赖氨酸。8千道尔顿的生长抑素28(1 - 12)样肽对应于不含精氨酸 - 赖氨酸 - 生长抑素 - 14的整个前生长抑素分子。较小的肽（5千道尔顿）由44个氨基酸组成，是在前生长抑素第56 - 57位的亮氨酸 - 亮氨酸键断裂后产生的。8千道尔顿和5千道尔顿的生长抑素28(1 - 12)样肽在其C末端均含有生长抑素28(1 - 12)。目前已鉴定的源自前生长抑素（前 - proSS）的4种最丰富的神经肽为：生长抑素 - 14、生长抑素28(1 - 12)、生长抑素28和前 - proSS25 - 100。
