Cremaschi D, James P S, Meyer G, Smith M W
Comp Biochem Physiol A Comp Physiol. 1984;78(4):661-6. doi: 10.1016/0300-9629(84)90613-3.
A technique is described allowing microelectrode impalement of enterocytes located at known positions along intestinal villi from rabbits and hamsters. Using this technique a 5 mV hyperpolarization in membrane potential is shown to occur as enterocytes migrate over the basal third of intestinal villi. The villus structure of the hamster ileum is similar to the rabbit, but the enterocyte lifespan in these two tissues differs considerably (enterocyte migration rates of 17.6 and 6.3 microns hr-1 for hamster and rabbit respectively). A correlation was found between the position an enterocyte occupied on the crypt-villus axis and the developmental state of the membrane potential. No such correlation existed when making comparisons on a time basis. These results are discussed both in terms of what is now known concerning different aspects of enterocyte development and in relation to what type of control mechanism might be generally responsible for initiating differentiation in this tissue.
本文描述了一种技术，该技术可对位于兔和仓鼠肠道绒毛上已知位置的肠上皮细胞进行微电极穿刺。使用该技术发现，当肠上皮细胞迁移至肠道绒毛基部三分之一区域时，膜电位会出现5 mV的超极化。仓鼠回肠的绒毛结构与兔相似，但这两种组织中肠上皮细胞的寿命差异很大（仓鼠和兔的肠上皮细胞迁移速率分别为17.6和6.3微米/小时）。研究发现，肠上皮细胞在隐窝-绒毛轴上占据的位置与膜电位的发育状态之间存在相关性。但在基于时间进行比较时，不存在这种相关性。本文从目前已知的肠上皮细胞发育的不同方面，以及可能普遍负责启动该组织分化的控制机制类型这两个角度对这些结果进行了讨论。
