Smith M W, Patterson J Y, Peacock M A
Comp Biochem Physiol A Comp Physiol. 1984;77(4):655-62. doi: 10.1016/0300-9629(84)90180-4.
Pieces of small intestine taken from rabbit, rat, mouse, guinea-pig, hamster and pig have been used to determine microvillus length in enterocytes located at different points along the crypt-villus axis. Thymidine labelling has also been used to convert measurements of enterocyte position into age of enterocyte. Microvillus lengths showed lower and upper plateaux (a and a + c respectively) with fairly rapid transition from one to the other defined by an exponential coefficient b. The mid-point of elongation (m) usually occurred within 60 micron of the crypt-villus junction. Correlations were found to exist between a and m and between a, m and the depth of the intestinal crypt. Values of b and bc/4, the maximal rate of microvillus elongation, were also found to be correlated with the size of the crypt. None of these parameters were related in any way to the villus height or enterocyte turnover time. The possibility that some factor associated with the physical size of the crypt might be exerting positional and temporal control over the subsequent structural differentiation of enterocytes is discussed.
取自兔、大鼠、小鼠、豚鼠、仓鼠和猪的小肠片段已被用于测定沿隐窝 - 绒毛轴不同位置的肠上皮细胞中的微绒毛长度。胸腺嘧啶核苷标记也已被用于将肠上皮细胞位置的测量值转化为肠上皮细胞的年龄。微绒毛长度呈现出较低和较高的平稳期（分别为a和a + c），从一个平稳期到另一个平稳期的转变相当迅速，由指数系数b定义。伸长的中点（m）通常出现在隐窝 - 绒毛交界处的60微米范围内。发现a与m之间以及a、m与肠隐窝深度之间存在相关性。还发现b和bc/4（微绒毛伸长的最大速率）的值与隐窝大小相关。这些参数均与绒毛高度或肠上皮细胞更新时间无关。本文讨论了与隐窝物理大小相关的某些因素可能对肠上皮细胞随后的结构分化施加位置和时间控制的可能性。
