Seifert J, Casida J E
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;78(2):271-6. doi: 10.1016/0742-8413(84)90082-3.
At least three forms of phenyl valerate esterases are present in hen brain cytoplasmic microtubules (MT). Thermostability studies reveal two additional forms in brain homogenates of cow, mouse, pig, rabbit and rat. The distribution of these brain esterases is not related to the age of the hens or the susceptibility of the species to organophosphate (OP)-induced delayed neurotoxicity. MT phenyl valerate esterases are distinct enzymes from the MT-associated proteases degrading high-molecular weight MT-associated proteins (hmw MAPs). Hen brain and spinal cord lysosomes on in vitro incubation release phenyl valerate esterase(s) and hmw MAPs-protease(s). OP neurotoxicants act in vitro to stabilize rat but not hen brain lysosomes. In vivo studies with hen brain and spinal cord lysosomes indicate that OP-induced delayed neurotoxicity is not initiated by disruption of lysosomal stability.
鸡脑细胞质微管（MT）中至少存在三种形式的苯戊酸酯酶。热稳定性研究表明，在牛、小鼠、猪、兔和大鼠的脑匀浆中还存在另外两种形式。这些脑酯酶的分布与母鸡的年龄或物种对有机磷酸酯（OP）诱导的迟发性神经毒性的易感性无关。MT苯戊酸酯酶与降解高分子量MT相关蛋白（hmw MAPs）的MT相关蛋白酶是不同的酶。鸡脑和脊髓溶酶体在体外孵育时会释放苯戊酸酯酶和hmw MAPs蛋白酶。OP神经毒物在体外作用于稳定大鼠而非鸡脑溶酶体。对鸡脑和脊髓溶酶体的体内研究表明，OP诱导的迟发性神经毒性不是由溶酶体稳定性破坏引发的。
