Michels E, Stenzinger W, Sorg C
Eur J Immunol. 1984 Oct;14(10):902-5. doi: 10.1002/eji.1830141008.
Mouse bone marrow cells differentiate in culture in the presence of L cell-conditioned medium to macrophages (M phi). Proliferation, release of plasminogen activator, expression of transglutaminase, random motility and response to a standard preparation of purified M phi migration inhibitory factor (MIF) was recorded daily up to 14 days. After an initial phase of proliferation, precursor cells differentiated into M phi. In the course of maturation, plasminogen activator production was transiently expressed between day 4 and 12; beginning on day 5 the cells expressed intracellular transglutaminase. Random motility of cells was high at the beginning of culture but steadily declined thereafter. The response to MIF was only expressed between day 5 and 8. However, it was possible to induce MIF responsiveness in mature, unresponsive M phi by the addition of L cell-conditioned medium. To characterize the MIF-responsive M phi type further, bone marrow-derived M phi at day 6 of culture were separated on a hypotonic Percoll gradient into three distinct cell bands. While all densities of M phi displayed random migration, only cells with a density between 1.060 and 1.065 were responsive to MIF. We conclude that the response of M phi to MIF is a phenotypic trait transiently expressed in the course of maturation.
在L细胞条件培养基存在的情况下，小鼠骨髓细胞在培养中可分化为巨噬细胞（M phi）。每天记录细胞增殖、纤溶酶原激活物的释放、转谷氨酰胺酶的表达、随机运动以及对纯化的巨噬细胞迁移抑制因子（MIF）标准制剂的反应，持续14天。经过初始增殖阶段后，前体细胞分化为M phi。在成熟过程中，纤溶酶原激活物的产生在第4天至第12天短暂表达；从第5天开始，细胞表达细胞内转谷氨酰胺酶。培养开始时细胞的随机运动性较高，但此后稳步下降。对MIF的反应仅在第5天至第8天表达。然而，通过添加L细胞条件培养基，可以在成熟的、无反应的M phi中诱导出MIF反应性。为了进一步表征对MIF有反应的M phi类型，在培养第6天从骨髓来源的M phi在低渗Percoll梯度上分离成三个不同的细胞带。虽然所有密度的M phi都表现出随机迁移，但只有密度在1.060至1.065之间的细胞对MIF有反应。我们得出结论，M phi对MIF的反应是在成熟过程中短暂表达的一种表型特征。
