用于测定促甲状腺素受体抗体的冷冻保存人甲状腺组织：与新鲜组织的比较

Cryopreserved human thyroid tissue for TSAb determination: comparison with fresh tissue.

作者信息

Tanaka-Matsuura L M, Werner R S, Farah C S, Romaldini J H

出版信息

Horm Res. 1984;20(2):124-8. doi: 10.1159/000179984.

Abstract

A thyroid-stimulating antibody assay using human normal thyroid slices was performed for studying whether the cryopreservation technique produced a sensitive substrate for cyclic-AMP generation. Cryopreservation was carried out in sterile 15% glycerol 0.9% NaCl placed in a hexane-nitrogen bath at -196 degrees C and stored at -80 degrees C for 1-5 weeks. Dose-response curves for untreated Graves' hyperthyroid patients IgG and bovine TSH were similar using fresh and cryopreserved slices. Assay sensibility was 5 mU/ml TSH. A high correlation (r = 0.91; p less than 0.001; n = 41) was found for percentage of cyclic-AMP increase to b-TSH and Graves' IgG comparing both methods. Studying 19 Graves' hyperthyroid patients, positive results were found in 63 and 52% for fresh and cryopreserved slices respectively. The cryopreservation technique used in the study was efficient for TSAb determinations.

摘要

为研究冷冻保存技术是否能产生用于环磷腺苷生成的敏感底物，进行了一项使用人正常甲状腺切片的促甲状腺素抗体检测。冷冻保存在置于-196℃己烷-氮浴中的无菌15%甘油0.9%氯化钠中进行，并在-80℃储存1至5周。使用新鲜和冷冻保存的切片时，未经治疗的格雷夫斯甲亢患者IgG和牛促甲状腺素的剂量反应曲线相似。检测灵敏度为5 mU/ml促甲状腺素。比较两种方法时，发现环磷腺苷增加百分比与b-促甲状腺素和格雷夫斯IgG之间具有高度相关性（r = 0.91；p < 0.001；n = 41）。研究19例格雷夫斯甲亢患者，新鲜和冷冻保存切片的阳性结果分别为63%和52%。该研究中使用的冷冻保存技术对促甲状腺素受体抗体的测定是有效的。